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Mechanism-oriented redesign of an isomaltulose synthase to an isomelezitose synthase by site-directed mutagenesis.
Julian Görl, Malte Timm, Jürgen Seibel
Institute of Organic Chemistry, Julius-Maximilians University Würzburg, Würzburg, Germany.
Chembiochem : a European journal of chemical biology 2012 Jan 2Sizes of these terms reflect their relevance to your search.
An isomelezitose synthase was redesigned out of the sucrose isomerase from Protaminobacter rubrum for the synthesis of isomelezitose (6-O(F)-glucosylsucrose), a potential nutraceutical. The variants F297A, F297P, R333K, F321A_F319A and E428D catalyze the formation of isomelezitose in up to 70 % yield. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Julian Görl, Malte Timm, Jürgen Seibel. Mechanism-oriented redesign of an isomaltulose synthase to an isomelezitose synthase by site-directed mutagenesis. Chembiochem : a European journal of chemical biology. 2012 Jan 2;13(1):149-56
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PMID: 22124943
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