Raquel Di Falco Cossiello, Alexandros Aggelis, Daniel Faúndes, Carlos A Petta
Campinas Center of Human Reproduction, Campinas, São Paulo, Brazil.
Zygote (Cambridge, England) 2012 NovTo compare the effects of four culture media on the quality of human zygotes and embryos. Prospective study analyzing 2289 human embryos cultivated simultaneously in two different culture media: HTF, the default medium, with either Universal IVF, Global or IVF-30 as the secondary media. The sibling oocytes by each patient were randomly divided between the two culture media following intracytoplasmic sperm injection (ICSI). On day 1 the pronuclear stage of zygotes were evaluated and on day 2 embryos were evaluated according to the number of cells, percentage of fragmentation and number of nuclei. Z-test and odds ratios were used in the statistical analysis. There was a higher percentage (55.2%) of class A1 + A2 zygotes with IVF-30 compared with HTF, Global or Universal IVF media (49.1%, 44.7% and 44.2%, respectively). The percentage of Top embryos was significantly higher with Global (40.2%) compared with HTF (21.3%), IVF-30 (25.0%) or Universal IVF media (11.2%). Global medium produced more Top embryos evaluated on day 2 of development.
Raquel Di Falco Cossiello, Alexandros Aggelis, Daniel Faúndes, Carlos A Petta. Morphological differences in human zygotes and embryos cultured in different media. Zygote (Cambridge, England). 2012 Nov;20(4):399-405
PMID: 22126911
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