Isolation of primary avian osteocytes.

Cor M Semeins, Astrid D Bakker, Jenneke Klein-Nulend

Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands. c.semeins@acta.nl

Methods in molecular biology (Clifton, N.J.) 2012

filter terms:

Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.

Citation

Cor M Semeins, Astrid D Bakker, Jenneke Klein-Nulend. Isolation of primary avian osteocytes. Methods in molecular biology (Clifton, N.J.). 2012;816:43-53