Ubiquitin homeostasis is critical for synaptic development and function.

The ubiquitin-proteasome system (UPS) controls protein abundance and is essential for many aspects of neuronal function. In ataxia (ax(J)) mice, profound neurological and synaptic defects result from a loss-of-function mutation in the proteasome-associated deubiquitinating enzyme Usp14, which is required for recycling ubiquitin from proteasomal substrates. Here, we show that transgenic complementation of ax(J) mice with neuronally expressed ubiquitin prevents early postnatal lethality, restores muscle mass, and corrects developmental and functional deficits resulting from the loss of Usp14, demonstrating that ubiquitin deficiency is a major cause of the neurological defects observed in the ax(J) mice. We also show that proteasome components are normally induced during the first 2 weeks of postnatal development, which coincides with dramatic alterations in polyubiquitin chain formation. These data demonstrate a critical role for ubiquitin homeostasis in synaptic development and function, and show that ubiquitin deficiency may contribute to diseases characterized by synaptic dysfunction.

Citation

Ping-Chung Chen, Bula J Bhattacharyya, John Hanna, Heather Minkel, Julie A Wilson, Daniel Finley, Richard J Miller, Scott M Wilson. Ubiquitin homeostasis is critical for synaptic development and function. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 Nov 30;31(48):17505-13

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PMID: 22131412

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