Capillary electrophoresis and 5-channel LIF detection of a 26plex autosomal STR assay for human identification.

Division of Biochemical Science, National Institute of Standards and Technology, Gaithersburg, MD, USA. becky.hill@nist.gov

Methods in molecular biology (Clifton, N.J.) 2012

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Multiplex polymerase chain reaction (PCR) is a common method used for DNA typing in forensic and paternity cases. There are numerous commercial short tandem repeat (STR) multiplex assays currently available to the forensic community. These assays amplify the core Combined DNA Index System (CODIS) STR loci for entry into the US. DNA database. Additional non-CODIS loci, which are considered genetically unlinked to the CODIS loci, can be useful in resolving challenging cases such as missing persons and mass disaster victim identification, paternity testing, and immigration testing. An STR multiplex has been successfully developed with 25 non-CODIS autosomal loci plus the sex-typing locus amelogenin for a total of 26 loci in a single 26plex amplification reaction. This chapter will focus on the preparation and the use of the 26plex assay with DNA samples for the purpose of human identification.

Citation

Carolyn R Hill. Capillary electrophoresis and 5-channel LIF detection of a 26plex autosomal STR assay for human identification. Methods in molecular biology (Clifton, N.J.). 2012;830:17-29