Correlation Engine 2.0
Clear Search sequence regions

Sizes of these terms reflect their relevance to your search.

High-throughput DNA sequencing technologies are increasingly becoming powerful systems for the comprehensive analysis of variations in whole genomes or various DNA libraries. As they are capable of producing massive collections of short sequences with varying lengths, a major challenge is how to turn these reads into biologically meaningful information. The first stage is to assemble the short reads into longer sequences through an in silico process. However, currently available software/programs allow only the assembly of abundant sequences, which apparently results in the loss of highly variable (or rare) sequences or creates artefact assemblies. In this paper, we describe a novel program (DNAseq) that is capable of assembling highly variable sequences and displaying them directly for phylogenetic analysis. In addition, this program is Microsoft Windows-based and runs by a normal PC with 700MB RAM for a general use. We have applied it to analyse a human naive single-chain antibody (scFv) library, comprehensively revealing the diversity of antibody variable complementarity-determining regions (CDRs) and their families. Although only a scFv library was exemplified here, we envisage that this program could be applicable to other genome libraries. Copyright © 2011 Elsevier B.V. All rights reserved.


Klaus S Lassen, Henrik Schultz, Niels H H Heegaard, Mingyue He. A novel DNAseq program for enhanced analysis of Illumina GAII data: a case study on antibody complementarity-determining regions. New biotechnology. 2012 Feb 15;29(3):271-8

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 22155428

View Full Text