A one-step affinity-purification protocol to purify NB-LRR immune receptors from plants that mediate resistance to fungal pathogens.

Wladimir I L Tameling

Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands. wladimir.tameling@wur.nl

Methods in molecular biology (Clifton, N.J.) 2012

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Nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors from plants confer resistance to fungal pathogens and many other pathogenic organisms. Their low expression makes it challenging to purify these receptors from plants in sufficient quantities to be able to identify interacting proteins by mass spectrometry. Here we describe a protocol to affinity-purify recombinant NB-LRR immune receptors, fused to the streptavidin-binding peptide tag.

Citation

Wladimir I L Tameling. A one-step affinity-purification protocol to purify NB-LRR immune receptors from plants that mediate resistance to fungal pathogens. Methods in molecular biology (Clifton, N.J.). 2012;835:585-90