Isolation and characterization of naïve follicular dendritic cells.

Kenta Usui, Shin-ichiro Honda, Yuichi Yoshizawa, Chigusa Nakahashi-Oda, Satoko Tahara-Hanaoka, Kazuko Shibuya, Akira Shibuya

Department of Immunology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Ten-nodai, Tsukuba, Ibaraki 305-8575, Japan.

Molecular immunology 2012 Mar

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Follicular dendritic cells (FDC) are specialized antigen-presenting cells to cognate B cells in the follicle of the lymphoid tissues. FDC also support survival and proliferation of the B cells, leading to the germinal center formation. FDC therefore play a central role in humoral immune responses. However, molecular and functional characteristics of FDC are largely unknown, because it is difficult to isolate and analyze FDC due to a very small number of FDC in the lymphoid tissues and the fragility by mechanical and chemical stresses in vitro. In this report, we established a novel method for FDC isolation from the spleen of naïve mice by flow cytometry and analyzed the phenotypical and functional characteristics. The isolated FDC, which accounted for ~0.2% of the spleen cells of naïve mice, were CD45(-), FDC-M2(+), and ICAM-1(+), and supported the survival and LPS-induced proliferation of B cells. We also showed that a neutralizing antibody against B cell activating factor TNF family (BAFF) suppressed FDC-dependent B cell proliferation in the presence of LPS, but not survival, demonstrating the evidence that FDC-derived BAFF is involved in B cell proliferation. Copyright Â© 2011 Elsevier Ltd. All rights reserved.

Citation

Kenta Usui, Shin-ichiro Honda, Yuichi Yoshizawa, Chigusa Nakahashi-Oda, Satoko Tahara-Hanaoka, Kazuko Shibuya, Akira Shibuya. Isolation and characterization of naïve follicular dendritic cells. Molecular immunology. 2012 Mar;50(3):172-6