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A method is described for construction of an amperometric polyphenol biosensor employing nitrocellulose membrane-bound laccase purified from cell-free extract of Ganoderma lucidum onto a Pt electrode. The biosensor showed optimum response within 10s, at 0.4 V in 0.1M acetate buffer, pH 6.0, and 35°C. Detection limit of the biosensor was 3.0 × 10(-8)M. Analytical recovery of added guaiacol was 97.00%. Within batch and between batch coefficients of variation were <0.97% and <1.26%, respectively. The sensor measured total phenolic content in fruit juices and alcoholic beverages. The enzyme electrode was used 100 times over 4 months, when stored at 4°C.

Citation

Chandra Shekhar Pundir, Rachna Rawal, Sheetal Chawla, Renuka, Ramesh Chandra Kuhad. Development of an amperometric polyphenol biosensor based on fungal laccase immobilized on nitrocellulose membrane. Artificial cells, blood substitutes, and immobilization biotechnology. 2012 Feb;40(1-2):163-70

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PMID: 22192070

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