The cytosolic domain of human Tom22 modulates human Bax mitochondrial translocation and conformation in yeast.

Thibaud T Renault, Xavier Grandier-Vazeille, Hubert Arokium, Gisèle Velours, Nadine Camougrand, Muriel Priault, Oscar Teijido, Laurent M Dejean, Stéphen Manon

FEBS letters 2012 Jan 20

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The role of the mitochondrial protein receptor Tom22p in the interaction of pro-apoptotic protein Bax with yeast mitochondria was investigated. Co-immunoprecipitation assays showed that human Bax interacted with different TOM subunits, including Tom22p. Expression of the cytosolic receptor domain of human Tom22 increased Bax mitochondrial localization, but decreased the proportion of active Bax. BN-PAGE showed that the cytosolic domain of Tom22 interfered with the oligomerization of Bax. These data suggest that the interaction with the cytosolic domain of Tom22 helps Bax to acquire a conformation able to interact with the outer mitochondrial membrane. Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Thibaud T Renault, Xavier Grandier-Vazeille, Hubert Arokium, Gisèle Velours, Nadine Camougrand, Muriel Priault, Oscar Teijido, Laurent M Dejean, Stéphen Manon. The cytosolic domain of human Tom22 modulates human Bax mitochondrial translocation and conformation in yeast. FEBS letters. 2012 Jan 20;586(2):116-21