BVPaP-3, a T7-like lytic phage of Pseudomonas aeruginosa: its isolation and characterisation.

The increasing emergence of antibiotic-resistant bacteria has produced a growing interest among scientists in bacteriophages as alternative antimicrobial agents. This article reports a lytic phage against an antibiotic-resistant strain of Pseudomonas aeruginosa. Phage BVPaP-3 is a member of the Podoviridae family and morphologically similar to the T7-like phage gh-1. The phage has a hexagonal head of 58-59 nm in diameter and a short tail of 10 × 8 nm. It is stable at a wide range of pH (6-10) and temperatures (4-40°C). Its optimal growth temperature is 37°C and the adsorption rate constant is 1.19 × 10(-9). Latent and eclipse periods are 20 and 15 min, respectively, and the burst size is 44 after 35 min at 37°C. The phage has a DNA size of 41.31 kb and a proteome of 11 proteins. The major protein is 33 kDa in size.

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Sangeeta Ahiwale, Divya Prakash, Milind Gajbhiye, Smita Jagdale, Nita Patil, Balu Kapadnis. BVPaP-3, a T7-like lytic phage of Pseudomonas aeruginosa: its isolation and characterisation. Current microbiology. 2012 Apr;64(4):305-11

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PMID: 22215482

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