Reversible regulation of protein binding affinity by a DNA machine.

Chao Zhou, Zhongqiang Yang, Dongsheng Liu

Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China.

Journal of the American Chemical Society 2012 Jan 25

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We report a DNA machine that can reversibly regulate target binding affinity on the basis of distance-dependent bivalent binding. It is a tweezer-like DNA machine that can tune the spatial distance between two ligands to construct or destroy the bivalent binding. The DNA machine can strongly bind to the target protein when the ligands are placed at an appropriate distance but releases the target when the bivalent binding is disrupted by enlargement of the distance between the ligands. This "capture-release" cycle could be repeatedly driven by single-stranded DNA without changing the ligands and target protein. © 2012 American Chemical Society

Citation

Chao Zhou, Zhongqiang Yang, Dongsheng Liu. Reversible regulation of protein binding affinity by a DNA machine. Journal of the American Chemical Society. 2012 Jan 25;134(3):1416-8