Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane.

Mathias J Gerl, Julio L Sampaio, Severino Urban, Lucie Kalvodova, Jean-Marc Verbavatz, Beth Binnington, Dirk Lindemann, Clifford A Lingwood, Andrej Shevchenko, Cornelia Schroeder, Kai Simons

Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.

The Journal of cell biology 2012 Jan 23

filter terms:

The influenza virus (IFV) acquires its envelope by budding from host cell plasma membranes. Using quantitative shotgun mass spectrometry, we determined the lipidomes of the host Madin-Darby canine kidney cell, its apical membrane, and the IFV budding from it. We found the apical membrane to be enriched in sphingolipids (SPs) and cholesterol, whereas glycerophospholipids were reduced, and storage lipids were depleted compared with the whole-cell membranes. The virus membrane exhibited a further enrichment of SPs and cholesterol compared with the donor membrane at the expense of phosphatidylcholines. Our data are consistent with and extend existing models of membrane raft-based biogenesis of the apical membrane and IFV envelope.

Citation

Mathias J Gerl, Julio L Sampaio, Severino Urban, Lucie Kalvodova, Jean-Marc Verbavatz, Beth Binnington, Dirk Lindemann, Clifford A Lingwood, Andrej Shevchenko, Cornelia Schroeder, Kai Simons. Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane. The Journal of cell biology. 2012 Jan 23;196(2):213-21