BaseSpace
Correlation Engine 2.0
Import Your Data
Literature

FAQ

More FAQs

Back to top
Clear Search sequence regions
(e.g. Trichostatin A, rs2300478, TGFB1, Apoptosis, Allergy to pollen, Neuron, Nosology)
Bookmark Forward

Efficient purification of native recombinant proteins using proteases immobilized on cellulose.

Dongming Lan, Yan Tai, Yanfei Shen, Fanghua Wang, Bo Yang, Yonghua Wang

School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, PR China.

Journal of bioscience and bioengineering 2012 Apr


filter terms:
  • cellulose (4)
  • cellulose binding (4)
  • centrifugation (1)
  • enzymes immobilized (2)
  • green fluorescent protein (3)
  • native (1)
  • protease (5)
  • protein binding (1)
  • protein engineering (1)
  • proteins immobilized (2)
  • recombinant proteins (3)
    • Sizes of these terms reflect their relevance to your search.

    Cellulose binding domain (CBD) fusion protease was employed to digest the CBD fusion protein. After cleavage, the tag free target proteins can be separated from the CBD tag and CBD fusion protease which still adsorbed to the cellulose by centrifugation. The green fluorescent protein was efficiently purified using this method. Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

    Citation

    Dongming Lan, Yan Tai, Yanfei Shen, Fanghua Wang, Bo Yang, Yonghua Wang. Efficient purification of native recombinant proteins using proteases immobilized on cellulose. Journal of bioscience and bioengineering. 2012 Apr;113(4):542-4

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 22265893

    View Full Text
    • Copyright © 2024 Illumina Inc. All rights reserved.