Chiral separation of vinpocetine using cyclodextrin-modified micellar electrokinetic chromatography.

Wan Aini Wan Ibrahim, Siti Munirah Abd Wahib, Dadan Hermawan, Mohd Marsin Sanagi, Hassan Y Aboul-Enein

Separation Science and Technology Group, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, Malaysia. wanaini@kimia.fs.utm.my

Chirality 2012 Mar

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A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique has been developed for enantioseparation of vinpocetine using an inexpensive 2-hydroxypropyl-β-CD (HP-β-CD) as the chiral selector (CS). The best chiral separation was achieved using 40 mM HP-β-CD as the CS in 50 mM phosphate buffer (pH 7.0) consisting of 40 mM sodium dodecyl sulfate (SDS) at a separation temperature and separation voltage of 25°C and 25 kV, respectively. To the author's best knowledge, this is the first CD-MEKC study able to successfully separate the four stereoisomer of vinpocetine in separation time of 9.5 min and resolution of 1.04-3.87. Copyright © 2012 Wiley Periodicals, Inc.

Citation

Wan Aini Wan Ibrahim, Siti Munirah Abd Wahib, Dadan Hermawan, Mohd Marsin Sanagi, Hassan Y Aboul-Enein. Chiral separation of vinpocetine using cyclodextrin-modified micellar electrokinetic chromatography. Chirality. 2012 Mar;24(3):252-4