Influence of peptide derivatives of diketopiperazines on hemopoietic stem cells of an intact organism and irradiated bone marrow cells in vitro].

V I Deĭgin, A S Saenko, Iu A Semin, A S Shevchenko, A A Luzianina, O S Izmest'eva, L P Zhavoronkov

Radiatsionnaia biologiia, radioecologiia / Rossiĭskaia akademiia nauk 2011 Sep-Oct

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We have performed structural and functional studies of the hemotropic activity for a number of novel 2,5-diketopiperazine peptidomimetic derivatives. We employed a mouse model of hemopoietic stem cells cloning in the spleen of lethally irradiated animals. Biologic activity of synthetic products was studied in two experimental models: 1) in vitro irradiated bone marrow SFU-S was used for studying the radio modifying activity; 2) the biological effect of peptidomimetics on the intact non-irradiated bone marrow was evaluated in vivo. Various ways of administration of the peptidomimetics studied were used in the in vivo experiments: intravenous, intraperitoneal or subcutaneous injections and oral administration in the dose range of 10-10000 microg/kg. As a result of our work, we have discovered 2,5-diketopiperazine peptidomimetic derivatives with the dual activity: stimulation of intact committed SFU-S and radiomodifying activity.

Citation

V I Deĭgin, A S Saenko, Iu A Semin, A S Shevchenko, A A Luzianina, O S Izmest'eva, L P Zhavoronkov. Influence of peptide derivatives of diketopiperazines on hemopoietic stem cells of an intact organism and irradiated bone marrow cells in vitro]. Radiatsionnaia biologiia, radioecologiia / Rossiĭskaia akademiia nauk. 2011 Sep-Oct;51(5):530-5