Flow-injection chemiluminescence determination of haemoglobin in the blood.

Zoumana Sekou Traore, Syed Mazhar Shah, Xingguang Su

Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, People's Republic of China.

Luminescence : the journal of biological and chemical luminescence 2013 Jan-Feb

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In this study, a sensitive and simple flow-injection chemiluminescence (CL) method was developed for the quantitative analysis of haemoglobin. The method is based on the ability of haemoglobin to enhance the CL signal generated by a H(2)O(2) -K(4) Fe(CN)(6) -fluorescein alkaline system enhanced by CdTe quantum dots. Under the optimized conditions, haemoglobin can be detected in concentration range 7.35 × 10(-9) -2.5 × 10(-6) mol/L, with a detection limit (3σ) of 1.8 × 10(-9) mol/L and a relative standard deviation (RSD; for 5 × 10(-7) mol/L haemoglobin) of 2.06% (n = 11). The present CL method was successfully applied for the determination of haemoglobin in three kinds of blood samples taken from an infant, an adult man, an adult woman and two reference samples. Compared with previous reports, the CL method described in this work is simple and rapid, with high sensitivity. Copyright © 2012 John Wiley & Sons, Ltd.

Citation

Zoumana Sekou Traore, Syed Mazhar Shah, Xingguang Su. Flow-injection chemiluminescence determination of haemoglobin in the blood. Luminescence : the journal of biological and chemical luminescence. 2013 Jan-Feb;28(1):56-62