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α-Glucuronidase enzymes play an essential role in the full enzymatic hydrolysis of hemicellulose. Up to this point, all genes encoding α-glucuronidase enzymes have been cloned from individual, pure culture strains. Using a high-throughput screening strategy, we have isolated the first α-glucuronidase gene (rum630-AG) from a mixed population of microorganisms. The gene was subcloned into a prokaryotic vector, and the enzyme was overexpressed and biochemically characterized. The RUM630-AG enzyme had optimum activity at pH 6.5 and 40 °C. When birchwood xylan was used as substrate, the RUM630-AG functioned synergistically with an endoxylanase enzyme to hydrolyze the substrate.

Citation

Charles C Lee, Rena E Kibblewhite, Kurt Wagschal, Ruiping Li, William J Orts. Isolation of α-glucuronidase enzyme from a rumen metagenomic library. The protein journal. 2012 Mar;31(3):206-11

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PMID: 22307653

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