Rapid identification of Enterococcus faecalis by species-specific primers based on the genes involved in the Entner-Doudoroff pathway.

Slavil Z Peykov, Vanya D Aleksandrova, Svetoslav G Dimov

Department of Genetics, Faculty of Biology, Sofia University St. Kliment Ohridski, Sofia, Bulgaria.

Molecular biology reports 2012 Jun

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In this study we report a novel method for identification of Enterococcus faecalis based on polymerase chain reaction with primers specific for the eda-genes encoding the enzymes involved in the Entner-Doudoroff pathway, a pathway present only in this species among Gram-positive bacteria. The designed primers were checked in several different Enterococcus species, and with some other Gram-positive and Gram-negative bacterial species as well. Five primer combinations were used to detect the eda-1 gene, and another three for the eda-2 gene. With the exception of one of the primer combinations, all the others gave as results the expected amplification products only in E. faecalis strains.

Citation

Slavil Z Peykov, Vanya D Aleksandrova, Svetoslav G Dimov. Rapid identification of Enterococcus faecalis by species-specific primers based on the genes involved in the Entner-Doudoroff pathway. Molecular biology reports. 2012 Jun;39(6):7025-30