Characterization of hammerhead ribozyme reactions.

Anne Kalweit, Rita Przybilski, Carsten Seehafer, Marcos de la Peña, Christian Hammann

Heisenberg Research Group Ribogenetics, Technical University of Darmstadt, Darmstadt, Germany.

Methods in molecular biology (Clifton, N.J.) 2012

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Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis.

Citation

Anne Kalweit, Rita Przybilski, Carsten Seehafer, Marcos de la Peña, Christian Hammann. Characterization of hammerhead ribozyme reactions. Methods in molecular biology (Clifton, N.J.). 2012;848:5-20