S Esin Çelik, Mustafa Ozyürek, Kubilay Güçlü, Reşat Apak
Department of Chemistry, Istanbul University, Avcilar, 34320 Istanbul, Turkey.
Analytical biochemistry 2012 Apr 1The cupric reducing antioxidant capacity (CUPRAC) method using bathocuproine sulfonate (BCS) as cuprous-chelating ligand claims to be an efficient substitute for the conventional CUPRAC assay using neocuproine; however, differences in their physical chemistry give rise to significantly varying responsivities to antioxidant compounds. The antioxidant capacity due to β-carotene (in acetone or dichloromethane) was fully measurable by conventional CUPRAC but not at all by cupric-BCS assay. Furthermore, CUPRAC exhibits distinctly stronger responses than cupric-BCS to naringenin, α-tocopherol, and bilirubin. Copyright © 2012 Elsevier Inc. All rights reserved.
S Esin Çelik, Mustafa Ozyürek, Kubilay Güçlü, Reşat Apak. Differences in responsivity of original cupric reducing antioxidant capacity and cupric-bathocuproine sulfonate assays to antioxidant compounds. Analytical biochemistry. 2012 Apr 1;423(1):36-8
PMID: 22326793
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