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The cupric reducing antioxidant capacity (CUPRAC) method using bathocuproine sulfonate (BCS) as cuprous-chelating ligand claims to be an efficient substitute for the conventional CUPRAC assay using neocuproine; however, differences in their physical chemistry give rise to significantly varying responsivities to antioxidant compounds. The antioxidant capacity due to β-carotene (in acetone or dichloromethane) was fully measurable by conventional CUPRAC but not at all by cupric-BCS assay. Furthermore, CUPRAC exhibits distinctly stronger responses than cupric-BCS to naringenin, α-tocopherol, and bilirubin. Copyright © 2012 Elsevier Inc. All rights reserved.


S Esin Çelik, Mustafa Ozyürek, Kubilay Güçlü, Reşat Apak. Differences in responsivity of original cupric reducing antioxidant capacity and cupric-bathocuproine sulfonate assays to antioxidant compounds. Analytical biochemistry. 2012 Apr 1;423(1):36-8

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PMID: 22326793

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