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Inactivation of recombinant bacteriophage lambda by use of chemical agents and UV radiation.

Ewan M Clark, Harry Wright, Kelly-Anne Lennon, Vicki A Craik, Jason R Clark, John B March

BigDNA Limited, Roslin, Midlothian, Scotland, United Kingdom.

Applied and environmental microbiology 2012 Apr


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    Several approaches for the inactivation of bacteriophage lambda, including UV germicidal irradiation (UVGI) and the chemical agents Virkon-S, Chloros, Decon-90, and sodium hydroxide (NaOH), were compared. Virkon, NaOH, and UVGI caused a ≥7-log(10) reduction in phage titers. This study successfully describes several methods with potential for bacteriophage inactivation in industrial settings.

    Citation

    Ewan M Clark, Harry Wright, Kelly-Anne Lennon, Vicki A Craik, Jason R Clark, John B March. Inactivation of recombinant bacteriophage lambda by use of chemical agents and UV radiation. Applied and environmental microbiology. 2012 Apr;78(8):3033-6

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    PMID: 22327583

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