The position of cleavage furrow in cultivated cells on the example of lines L-929 and CHO].

The aim of the study carried out on cells of lines L-929 (NCTC a clone 929) and CHO was to examine whether the position of cleavage furrow is random or not. CHO cells were seeded in the traditional way (evenly across the surface of a Petri dish), and L-cell were put as colonies to monitor the migration of cells emanating from them. The time-lapse filming (imaging) was used for registration of behavior of cells. Analyzing the obtained images of cells, the angle between the axis of polarization of a cell and its cleavage furrow was measured. Besides, the angle between an axis of cell polarization or its cleavage furrow and the horizontal axis of image field was measured. It was shown that position of CHO cells in a dish plane was random as well as the value of the angle between the axis of polarization of these cells and their cleavage furrows. The L-929 cells migrating from colony were orientated so, that their polarization axis was directed to the colony center, and the cleavage furrow was perpendicular with this axis. Assumptions about nonrandom position of both cultivated cells during mitosis and their cleavage furrows were made.

Citation

I u P Petrov, Iu A Neguliaev, N V Tsupkina. The position of cleavage furrow in cultivated cells on the example of lines L-929 and CHO]. Tsitologiia. 2011;53(11):839-47

Mesh Tags

PMID: 22332414

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