Mechanistic understanding of protein-silicone oil interactions.

Jinjiang Li, Swathi Pinnamaneni, Yong Quan, Archana Jaiswal, Fredrik I Andersson, Xiaochun Zhang

Pharmaceutical research 2012 Jun

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To investigate interactions between protein and silicone oil so that we can provide some mechanistic understanding of protein aggregation in silicone oil lubricated syringes and its prevention by formulation additives such as Polysorbate 80 and Poloxamer 188. Interfacial tension values of silicone oil/water interface of abatacept solutions with and without formulation additives were obtained under equilibrium conditions using Attension Theta optical tensiometer. Their adsorption and desorption profiles were measured using Quartz Crystal Microbalancing with Dissipation monitoring (QCM-D). The degree of aggregation of abatacept was assessed based on size exclusion measurement. Adsorption of abatacept at the oil/water interface was shown. Polysorbat 80 was more effective than Poloxamer 188 in preventing abatacept adsorption. Moreover, it was noted that some of the adsorbed abatacept molecules were not desorbed readily upon buffer rinse. Finally, no homogeneous aggregation was observed at room temperature and a slight increase of aggregation was only observed for samples measured at 40°C which can be prevented using Polysorbate 80. Interfacial adsorption of proteins is the key step and maybe responsible for the phenomenon of soluble-protein loss when contacting silicone oil and the irreversible adsorption of protein may be associated with protein denaturation/aggregation.

Citation

Jinjiang Li, Swathi Pinnamaneni, Yong Quan, Archana Jaiswal, Fredrik I Andersson, Xiaochun Zhang. Mechanistic understanding of protein-silicone oil interactions. Pharmaceutical research. 2012 Jun;29(6):1689-97