Detection of the early stage of recombinational DNA repair by silicon nanowire transistors.

Marco Chiesa, Paula P Cardenas, Francisco Otón, Javier Martinez, Marta Mas-Torrent, Fernando Garcia, Juan C Alonso, Concepció Rovira, Ricardo Garcia

Instituto de Microelectrónica de Madrid (IMM-CNM-CSIC), Isaac Newton 8, 28760 Tres Cantos, Madrid, Spain.

Nano letters 2012 Mar 14

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A silicon nanowire-based biosensor has been designed and applied for label-free and ultrasensitive detection of the early stage of recombinational DNA repair by RecA protein. Silicon nanowires transistors were fabricated by atomic force microscopy nanolithography and integrated into a microfluidic environment. The sensor operates by measuring the changes in the resistance of the nanowire as the biomolecular reactions proceed. We show that the nanoelectronic sensor can detect and differentiate several steps in the binding of RecA to a single-stranded DNA filament taking place on the nanowire-aqueous interface. We report relative changes in the resistance of 3.5% which are related to the interaction of 250 RecA·single-stranded DNA complexes. Spectroscopy data confirm the presence of the protein-DNA complexes on the functionalized silicon surfaces. © 2012 American Chemical Society

Citation

Marco Chiesa, Paula P Cardenas, Francisco Otón, Javier Martinez, Marta Mas-Torrent, Fernando Garcia, Juan C Alonso, Concepció Rovira, Ricardo Garcia. Detection of the early stage of recombinational DNA repair by silicon nanowire transistors. Nano letters. 2012 Mar 14;12(3):1275-81