Zwitterionic polycarboxybetaine coating functionalized with REDV peptide to improve selectivity for endothelial cells.

Ying Ji, Yu Wei, Xiangsheng Liu, Jinlei Wang, Kefeng Ren, Jian Ji

Department of Polymer Science and Engineering, MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Zhejiang University, Hangzhou 310027, People's Republic of China.

Journal of biomedical materials research. Part A 2012 Jun

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Surface immobilization of bioactive molecules has been a promising strategy to develop in situ endothelialization for cardiovascular implants. With the aim to construct endothelial cell specific coating with low fouling property, zwitterionic carboxybetaine methacrylate and butyl methacrylate were copolymerized as coating materials, spin-coated onto substrates, and immobilized with endothelial cell selective peptide Arg-Glu-Asp-Val (REDV) through functionalization of carboxy groups in carboxybetaine by NHS/EDC chemistry. Experimental results proved that carboxybetaine-REDV coating maintained desirable antifouling ability and fine hemocompatibility. Separate culture and coculture of HUVECs (human umbilical vein endothelial cells) with HUASMCs (human umbilical artery smooth muscle cells) showed that the coating was able to enhance the competitive growth of endothelial cells while limiting the adhesion, proliferation, and migration of smooth muscle cells. The existence of zwitterionic carboxybetaine helps to screen undesirable adsorption of platelets, and its nonspecific resistance to smooth muscle cells contributes to the realization of endothelial cell selectivity. Copyright © 2012 Wiley Periodicals, Inc.

Citation

Ying Ji, Yu Wei, Xiangsheng Liu, Jinlei Wang, Kefeng Ren, Jian Ji. Zwitterionic polycarboxybetaine coating functionalized with REDV peptide to improve selectivity for endothelial cells. Journal of biomedical materials research. Part A. 2012 Jun;100(6):1387-97