The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK.

Hilda Sucipto, Fumitaka Kudo, Tadashi Eguchi

Department of Chemistry and Materials Science, Tokyo Institute of Technology, O-okayama, Meguro-ku, Japan.

Angewandte Chemie (International ed. in English) 2012 Apr 2

filter terms:

Mystery solved: using heterologous expression, the activities of two enzymes exclusively belonging to the kanamycin biosynthetic pathway have been identified in vitro. A distinctive reaction mechanism to produce kanamycin is proposed and the previously unknown catalytic deamination activity of KanJ dioxygenase is uncovered. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Hilda Sucipto, Fumitaka Kudo, Tadashi Eguchi. The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK. Angewandte Chemie (International ed. in English). 2012 Apr 2;51(14):3428-31