Ying Zhou, Lei Shi, Dan Zheng, Yong Wang
Department of Pathogenic Biology, Nanjing Medical University, Nanjing 210029, China.
Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control 2011 DecTo study the responses of regulatory T cells induced by Sepharose 4B beads mixed antigen. Mice were immunized with different dosages of antigens, and the proportions of regulatory T cells were examined by flow cytometry. The 3H-thymidin incorporation method was used to detect the inhibitory function of Tregs. In vitro, dendritic cells were pulsed with different antigens. The expressions of MHC II, CD40, CD80/86 molecules on dendritic cells and the proportions of regulatory T cells were examined by flow cytometry. The 3H-thymidin incorporation method was used for the determination of Tregs function. Compared with control groups, the proportion of regulatory T cells in mice immunized with Sepharose 4B beads mixed antigen was(11.48 +/- 4.12)%, and the difference was significant (P < 0.05). The inhibition of regulatory T cells in mice immunized with Sepharose 4B beads mixed antigen showed a stronger potential (cpm was 720 +/- 180.4). Meanwhile, the proportions of regulatory T cells were (17.0 +/- 80.57)% and (30.14 +/- 3.62)% when the CD4+ T cells were co-cultured with dendritic cells pulsed with Sepharose 4B beads mixed rSj22.6/26GST and OVA, respectively. Sepharose 4B beads mixed antigen could induce the regulatory T cells in vivo and in vitro.
Ying Zhou, Lei Shi, Dan Zheng, Yong Wang. Effects of Schistosoma 22.6 kDa/26 GST molecule mixed with Sepharose 4B beads on induction of CD4+CD25+ regulatory T cells]. Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control. 2011 Dec;23(6):648-52
PMID: 22379819
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