Stimulation of GluN receptors decreases the surface density of GluN1/GluN2B subunits in cultured neocortical interneurons.

Wolfgang Nörenberg, A Kerstin Lindemeyer, Thomas Wilmes, Helga Sobottka, Dieter K Meyer

Rudolf-Boehm-Institute of Pharmacology and Toxicology, University of Leipzig, Leipzig, Germany.

Journal of neurochemistry 2012 May

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Changes in the density of NMDA (GluN) receptors in the neuronal membrane are critical for plasticity, whereas malfunction of precisely regulated GluN receptor activity may be involved in neurotoxicity. In cultured rat neocortical interneurons, we have studied the regulation of the surface density of GluN1, GluN2A and GluN2B subunits. Application of 5 μMol NMDA for 24 h followed by a washout period of 24 h decreased the response of GluN receptors for at least 2 days. The reduction was caused by a decrease in the surface density of GluN1/GluN2B subunits, whereas GluN2A subunits remained unaffected. Our data indicate that long but reversible low level activation of GluN receptors can cause long-term changes in their subunit composition in cultured interneurons. © 2012 The Authors. Journal of Neurochemistry © 2012 International Society for Neurochemistry.

Citation

Wolfgang Nörenberg, A Kerstin Lindemeyer, Thomas Wilmes, Helga Sobottka, Dieter K Meyer. Stimulation of GluN receptors decreases the surface density of GluN1/GluN2B subunits in cultured neocortical interneurons. Journal of neurochemistry. 2012 May;121(4):587-96