Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro].

To investigate miR-20a expression in human glioma and normal brain tissues and its effect on the proliferation of glioma cells in vitro. The expression of miR-20a was detected in human normal brain tissues and glioma tissues by real-time RT-PCR. miR-20a mimics were synthesized and transfected into U251 cells via liposome, and the cell proliferation were detected using MTT assay and flow cytometry. The glioma tissues showed significantly up-regulated expression of miR-20a compared with normal brain tissues (P=0.035). The expression level of miR-20a was higher in high-grade than in low-grade gliomas. miR-20a mimics significantly enhanced the proliferation of U251 cells and the percentage of S-phase cells. miR-20a shows potent effect in promoting the growth of glioma cells, suggesting its important role in the pathogenesis of human glioma.

Citation

Xingjun Yao, Wei Li, Yuehua Wang, Zhengbin Ding, Wenzhong Hou, Minmin Zeng, Guangce Deng, Jinzhu Zhang, Hongfa Yang. Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 2012 Feb;32(2):198-201

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PMID: 22381757

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