Use of dry-milling derived thin stillage for producing eicosapentaenoic acid (EPA) by the fungus Pythium irregulare.

Yi Liang, Xuefei Zhao, Megan Strait, Zhiyou Wen

Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, USA.

Bioresource technology 2012 May

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This study was to explore the use of thin stillage, a major byproduct in dry milling corn-ethanol plants, for production of eicosapentaenoic acid (EPA) by the fungus Pythium irregulare. Thin stillage contains various compounds that were ideal for fungal growth. Thin stillage concentration and temperature played important roles in fungal growth and EPA production. When 50% thin stillage was used in a stepwise temperature shift culture process, the cell density reached 23 g/L at day 9 with EPA yield and productivity of 243 and 27 mg/L day, respectively. The fungal biomass contained 39% lipid, 28% protein, 30% carbohydrate, and 3% ash. The fungal culture also generated a nutrient-depleted liquid by removing organic compounds in the raw thin stillage. The results collectively showed a new use of thin stillage by feeding to the fungus P. irregulare for producing omega-3 fatty acids. Copyright Â© 2012 Elsevier Ltd. All rights reserved.

Citation

Yi Liang, Xuefei Zhao, Megan Strait, Zhiyou Wen. Use of dry-milling derived thin stillage for producing eicosapentaenoic acid (EPA) by the fungus Pythium irregulare. Bioresource technology. 2012 May;111:404-9