Acadl-SNP based genotyping assay for long-chain acyl-CoA dehydrogenase deficient mice.

Rita J Luther, Alvin J O Almodovar, Russell Fullerton, Philip A Wood

Molecular genetics and metabolism 2012 May

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The long-chain acyl-CoA dehydrogenase (LCAD) (Acadl=gene; LCAD=protein) deficient mouse model has been important in evaluating the role of mitochondrial fatty acid oxidation of long-chain fatty acids in metabolic disorders. The insertion vector-based gene targeting strategy used to generate this model has made it difficult to distinguish homozygous and heterozygous genotypes containing targeted Acadl alleles in LCAD-deficient mice. Herein, we describe the design and validation of Acadl SNP genotyping methods capable of distinguishing between heterozygous and homozygous LCAD-deficient mice. The Acadl SNP genotyping assays are effective at allelic discrimination of both C57BL/6 and 129 mouse strain-based Acadl alleles under conditions including, both low purity and quantity genomic DNA templates. This makes the method practical and provides the necessary tools for genotyping the LCAD-deficient mouse model. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Rita J Luther, Alvin J O Almodovar, Russell Fullerton, Philip A Wood. Acadl-SNP based genotyping assay for long-chain acyl-CoA dehydrogenase deficient mice. Molecular genetics and metabolism. 2012 May;106(1):62-7