Frédéric A Perras, Ilia Korobkov, David L Bryce
Department of Chemistry and Centre for Catalysis Research and Innovation, University of Ottawa, 10 Marie Curie Private, Ottawa, Ontario, Canada.
Physical chemistry chemical physics : PCCP 2012 Apr 14Obtaining definitive information concerning the coordination environment of sodium ions which balance the negative charges found in nucleotides is a challenging task. We show that high resolution 1D and 2D (23)Na NMR spectra of sodium nucleotides obtained in the solid state with the use of double-rotation (DOR) provide valuable structural information. Sensitive spin diffusion homonuclear correlation experiments are used to establish the relative proximities of various pairs of crystallographically distinct Na sites and to assign the spectral resonances. Additionally, the DOR sidebands are simulated to obtain coordination information which is complementary to that obtained using multiple-quantum magic-angle spinning NMR spectra. These experiments led us to discover a new hendecahydrate of deoxycytidine monophosphate (dCMP), the structure of which is confirmed via single-crystal X-ray diffraction. This hydrate crystallizes reproducibly when deuterated water is used exclusively in the preparation process.
Frédéric A Perras, Ilia Korobkov, David L Bryce. 23Na double-rotation NMR of sodium nucleotides leads to the discovery of a new dCMP hendecahydrate. Physical chemistry chemical physics : PCCP. 2012 Apr 14;14(14):4677-81
PMID: 22389051
View Full Text