Thromboresistance characterization of extruded nitric oxide-releasing silicone catheters.

Kagya A Amoako, Christopher Archangeli, Hitesh Handa, Terry Major, Mark E Meyerhoff, Gail M Annich, Robert H Bartlett

Department of Biomedical Engineering, University of Michigan, Ann Arbor, 48109-0686, USA.

ASAIO journal (American Society for Artificial Internal Organs : 1992) 2012 May-Jun

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Intravascular catheters used in clinical practice can activate platelets, leading to thrombus formation and stagnation of blood flow. Nitric oxide (NO)-releasing polymers have been shown previously to reduce clot formation on a number of blood contacting devices. In this work, trilaminar NO-releasing silicone catheters were fabricated and tested for their thrombogenicity. All catheters had specifications of L = 6 cm, inner diameter = 21 gauge (0.0723 cm), outer diameter = 12 gauge (0.2052 cm), and NO-releasing layer thickness = 200 ± 11 µm. Control and NO-releasing catheters were characterized in vitro for their NO flux and NO release duration by gas phase chemiluminescence measurements. The catheters were then implanted in the right and left internal jugular veins of (N = 6 and average weight = 3 kg) adult male rabbits for 4 hours thrombogenicity testing. Platelet counts and function, methemoglobin (metHb), hemoglobin (Hb), and white cell counts and functional time (defined as patency time of catheter) were monitored as measured outcomes. Nitric oxide-releasing catheters (N = 6) maintained an average flux above (2 ± 0.5) × 10(-10) mol/min/cm for more than 24 hours, whereas controls showed no NO release. Methemoglobin, Hb, white cell, and platelet counts and platelet function at 4 hours were not significantly different from baseline (α = 0.05). However, clots on controls were visibly larger and prevented blood draws at a significantly (p < 0.05) earlier time (2.3 ± 0.7 hours) into the experiment, whereas all NO-releasing catheters survived the entire 4 hours test period. Results indicate that catheter NO flux levels attenuated thrombus formation in a short-term animal model.

Citation

Kagya A Amoako, Christopher Archangeli, Hitesh Handa, Terry Major, Mark E Meyerhoff, Gail M Annich, Robert H Bartlett. Thromboresistance characterization of extruded nitric oxide-releasing silicone catheters. ASAIO journal (American Society for Artificial Internal Organs : 1992). 2012 May-Jun;58(3):238-46