Cell ingression and apical shape oscillations during dorsal closure in Drosophila.

Adam Sokolow, Yusuke Toyama, Daniel P Kiehart, Glenn S Edwards

Physics Department, Duke University, Durham, North Carolina, USA.

Biophysical journal 2012 Mar 7

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Programmed patterns of gene expression, cell-cell signaling, and cellular forces cause morphogenic movements during dorsal closure. We investigated the apical cell-shape changes that characterize amnioserosa cells during dorsal closure in Drosophila embryos with in vivo imaging of green-fluorescent-protein-labeled DE-cadherin. Time-lapsed, confocal images were assessed with a novel segmentation algorithm, Fourier analysis, and kinematic and dynamical modeling. We found two generic processes, reversible oscillations in apical cross-sectional area and cell ingression characterized by persistent loss of apical area. We quantified a time-dependent, spatially-averaged sum of intracellular and intercellular forces acting on each cell's apical belt of DE-cadherin. We observed that a substantial fraction of amnioserosa cells ingress near the leading edges of lateral epidermis, consistent with the view that ingression can be regulated by leading-edge cells. This is in addition to previously observed ingression processes associated with zipping and apoptosis. Although there is cell-to-cell variability in the maximum rate for decreasing apical area (0.3-9.5 μm(2)/min), the rate for completing ingression is remarkably constant (0.83 cells/min, r(2) > 0.99). We propose that this constant ingression rate contributes to the spatiotemporal regularity of mechanical stress exerted by the amnioserosa on each leading edge during closure. Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Citation

Adam Sokolow, Yusuke Toyama, Daniel P Kiehart, Glenn S Edwards. Cell ingression and apical shape oscillations during dorsal closure in Drosophila. Biophysical journal. 2012 Mar 7;102(5):969-79