Immuno-like assays and biomimetic microchips.

M C Moreno-Bondi, M E Benito-Peña, J L Urraca, G Orellana

Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain. mcmbondi@quim.ucm.es

Topics in current chemistry 2012

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Biomimetic assays with molecularly imprinted polymers (MIPs) are bound to be an alternative to the traditional immuno-analytical methods based on antibodies. This is due to the unique combination of advantages displayed by the artificial materials including the absence of animal inoculation and sacrifice, unnecessary hapten conjugation to a carrier protein for stimulated production, the possibility of manufacturing MIPs against toxic substances, excellent physicochemical stability, reusability, ease of storage, and recognition in organic media. If the selectivity and affinity of MIPs are increased, many more immuno-like assays will be developed using radioactive, enzymatic, colorimetric, fluorescent, chemiluminescent, or electrochemical interrogation methods. This chapter provides a comprehensive comparison between the bio- and biomimetic entities and their usage.

Citation

M C Moreno-Bondi, M E Benito-Peña, J L Urraca, G Orellana. Immuno-like assays and biomimetic microchips. Topics in current chemistry. 2012;325:111-64