Recombinant monoclonal antibody recognizes a unique epitope on varicella-zoster virus immediate-early 63 protein.

We previously constructed a recombinant monoclonal antibody (rec-MAb 63P4) that detects immediate-early protein IE63 encoded by varicella-zoster virus (VZV) in the cytoplasm of productively infected cells. Here, we used ORF63 truncation mutants to map the rec-MAb 63P4 binding epitope to amino acids 141 to 150 of VZV IE63, a region not shared with other widely used anti-IE63 antibodies, and found that the recombinant antibody does not bind to the simian IE63 counterpart.

Citation

Niklaus H Mueller, Nathan L Bos, Scott Seitz, Mary Wellish, Ravi Mahalingam, Don Gilden, Randall J Cohrs. Recombinant monoclonal antibody recognizes a unique epitope on varicella-zoster virus immediate-early 63 protein. Journal of virology. 2012 Jun;86(11):6345-9

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PMID: 22438547

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