Design of [(2-pyrimidinylthio)acetyl]benzenesulfonamides as inhibitors of human carbonic anhydrases.

Edita Čapkauskaitė, Asta Zubrienė, Lina Baranauskienė, Giedrė Tamulaitienė, Elena Manakova, Visvaldas Kairys, Saulius Gražulis, Sigitas Tumkevičius, Daumantas Matulis

European journal of medicinal chemistry 2012 May

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A series of [(2-pyrimidinylthio)acetyl]benzenesulfonamides were designed and synthesized. Their binding affinities as inhibitors of several recombinant human carbonic anhydrase (CA) isozymes were determined by isothermal titration calorimetry (ITC) and thermal shift assay (TSA). A group of compounds containing a chlorine atom in the benzenesulfonamide ring were found to exhibit higher selectivity but lower binding affinity toward tested CAs. The crystal structures of selected compounds in complex with CA II were determined to atomic resolution. Docking studies were performed to compare the binding modes of experimentally determined crystallographic structures with computational prediction of the pyrimidine derivative binding to CA II. Several compounds bound to select CAs with single-digit nanomolar affinities and could be used as leads for inhibitor development toward a select CA isozyme. Copyright © 2012 Elsevier Masson SAS. All rights reserved.

Citation

Edita Čapkauskaitė, Asta Zubrienė, Lina Baranauskienė, Giedrė Tamulaitienė, Elena Manakova, Visvaldas Kairys, Saulius Gražulis, Sigitas Tumkevičius, Daumantas Matulis. Design of [(2-pyrimidinylthio)acetyl]benzenesulfonamides as inhibitors of human carbonic anhydrases. European journal of medicinal chemistry. 2012 May;51:259-70