Colocalization and identification of interaction sites between IGFBP-3 and GalNAc-T14.

GalNAc-T14 was identified as a novel IGFBP-3 binding partner in previous studies. Here, we furtherly confirmed the interaction between them by confocal microscopy, and identified the binding domain and probable interaction sites of GalNAc-T14 with IGFBP-3. The result of subcellular localization indicated that GalNAc-T14 was distributed in the cytosol, whereas IGFBP-3 existed in the cytosol and nucleolus. Confocal analyses demonstrated that IGFBP-3 and GalNAc-T14 colocalized in the cytosol. The result from yeast two hybrid assay showed that the C terminus of GalNAc-T14 (408-552aa) was essential for the interaction between GalNAc-T14 and IGFBP-3, especially Tyr(408), Pro(409), and Glu(410) of GalNAc-T14 may play key roles in the interaction with IGFBP-3. In conclusion, these studies demonstrated that IGFBP-3 and GalNAc-T14 are colocalized in MCF-7 cells and confirmed the interaction between IGFBP-3 and GalNAc-T14. This interaction may play an important role in the functional regulation of IGFBP-3. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Chen Wu, Si-Si Ma, Jian-Feng Ge, Yuan-Yuan Wang, Huan-Na Tian, Xiao-Bo Liu, Bo Zhang, Fang-Ming Liu, Xiao-Kang Zhang, Qin-Jian Li. Colocalization and identification of interaction sites between IGFBP-3 and GalNAc-T14. Gene. 2012 May 15;499(2):347-51

Mesh Tags

Substances

PMID: 22441125

search → result