A method for determining alkaline phosphatase activity in marine phytoplankton using spectrofluorometry.

Melissa Blakely Peacock, Raphael M Kudela

Department of Ocean Sciences, University of California Santa Cruz, CA 95064, USA. mdbpeacock@gmail.com

Journal of microbiological methods 2012 Jun

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A method for determining relative percent intensity alkaline phosphatase activity (APA) using enzyme labeled fluorescence coupled with spectrofluorometry is presented. Compared to traditional microscopy and flow cytometry, we increase statistical power and reduce sample-handling issues. Combined with a biological standard, our method can quantify APA of natural plankton assemblages. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Melissa Blakely Peacock, Raphael M Kudela. A method for determining alkaline phosphatase activity in marine phytoplankton using spectrofluorometry. Journal of microbiological methods. 2012 Jun;89(3):209-12