The smallest active fragment of microtubule-associated protein 4 and its interaction with microtubules in phosphate buffer.

Yurika Hashi, Lisa Nagase, Kazuyuki Matsushima, Susumu Kotani

Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa, Japan. r201070258yd@kanagawa-u.ac.jp

Bioscience, biotechnology, and biochemistry 2012

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To analyze the interaction between microtubule-associated protein (MAP) 4 and microtubules physicochemically, a MAP4 active site fragment was designed for nuclear magnetic resonance (NMR) use. The fragment was bacterially expressed and purified to homogeneity. The buffer conditions for NMR were optimized to support microtubule assembly. The fragment was found to bind to microtubules under the optimized buffer conditions.

Citation

Yurika Hashi, Lisa Nagase, Kazuyuki Matsushima, Susumu Kotani. The smallest active fragment of microtubule-associated protein 4 and its interaction with microtubules in phosphate buffer. Bioscience, biotechnology, and biochemistry. 2012;76(3):601-4