Optimizing Saccharomyces cerevisiae induction regimes.

David Drew, Hyun Kim

Division of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial College, London, UK.

Methods in molecular biology (Clifton, N.J.) 2012

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Recombinant membrane protein yields can be optimized in Saccharomyces cerevisiae by adjusting the induction time and temperature and/or by the addition of chemical chaperones. Here we describe a protocol for assessing the importance of these parameters.