Disruption of yeast cells to isolate recombinant proteins.

Mohammed Jamshad, Richard A J Darby

School of Biosciences, University of Birmingham, Birmingham, West Midlands, UK.

Methods in molecular biology (Clifton, N.J.) 2012

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Yeast is a proven host for the production of recombinant proteins, which may be incorporated in cellular membranes or localized in subcellular compartments. In order to gain access to these proteins, cellular disruption is required to permit extraction, purification, and downstream analysis. Disruption can significantly impact the yield and quality of the biomaterial. We highlight several disruption techniques that are applicable to yeast cells ranging from mechanical to nonmechanical approaches. In all cases fast, efficient cellular disruption is desirable, that does not alter the protein chemically or physically and that generates material for downstream purification and analysis.

Citation

Mohammed Jamshad, Richard A J Darby. Disruption of yeast cells to isolate recombinant proteins. Methods in molecular biology (Clifton, N.J.). 2012;866:237-46