The effects of diketopiperazines from Callyspongia sp. on release of cytokines and chemokines in cultured J774A.1 macrophages.

Jin-Hui Chen, Xiang-Peng Lan, Yonghong Liu, Ai-Qun Jia

Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education of China, Nanjing Forestry University, Nanjing 210037, China.

Bioorganic & medicinal chemistry letters 2012 May 1

filter terms:

Diketopiperazines (DKPs) are a class of secondary metabolites that result from peptide bonds between two amino acids to form a lactam. Due to their rigid structure, chiral nature, and varied side chains, DKPs have been of research interest for their diverse bioactivities. However, little is known about whether DPKs stimulate the release of cytokine and chemokines in macrophage cells. The present aim was to study the effect of DKPs firstly isolated from sponge Callyspongia sp. on the release of several cytokines in murine macrophage-like cell line J774A.1 after stimulation in vitro, and their potential structure-activity relationship of five natural DKPs on four representative cytokines, interferon-γ (IFN-γ), pro-inflammatory (tumor necrosis factor, TNF-α), anti-inflammatory cytokine (interleukin-10, IL-10), and chemokine (monocyte chemoattractant protein-1, MCP-1). Results suggested that these five DKPs, especially DKP 1 bearing 3-hydroxyl-l-proline (l-Hyp), might be useful as a promising macrophage cytokines stimulator. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Jin-Hui Chen, Xiang-Peng Lan, Yonghong Liu, Ai-Qun Jia. The effects of diketopiperazines from Callyspongia sp. on release of cytokines and chemokines in cultured J774A.1 macrophages. Bioorganic & medicinal chemistry letters. 2012 May 1;22(9):3177-80