Negative staining of lipopolysaccharides on polyacrylamide gels by using eosin B.

Zhong-Xin Zhu, Wei-Tao Cong, Xuan Zhou, Mao Chen, Jung-Kap Choi, Li-Tai Jin, Xiao-Kun Li

Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou 325035, China.

Analytical biochemistry 2012 Jul 1

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A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, stained, and developed within 30 min to achieve transparent and colorless LPS bands under opaque gel matrix background. As low as 20 to 40 ng of total LPSs could be detected, which is 4-fold more sensitive than those of the widely used silver stain developed by Fomsgaard and coworkers and imidazole-zinc (IZ) negative stain. For its sensitivity and brevity, this stain may be a practical method for LPS determination in the routine laboratory. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Zhong-Xin Zhu, Wei-Tao Cong, Xuan Zhou, Mao Chen, Jung-Kap Choi, Li-Tai Jin, Xiao-Kun Li. Negative staining of lipopolysaccharides on polyacrylamide gels by using eosin B. Analytical biochemistry. 2012 Jul 1;426(1):1-3