Detection of cells captured with antigens on shear horizontal surface-acoustic-wave sensors.

Techniques to separate cells are widely applied in immunology. The technique to separate a specific antigen on a microfluidic platform involves the use of a shear horizontal surface-acoustic-wave (SH-SAW) sensor. With specific antibodies conjugated onto the surface of the SH-SAW sensors, this technique can serve to identify specific cells in bodily fluids. Jurkat cells, used as a target in this work, provide a model of cells in small abundance (1:1000) for isolation and purification with the ultimate goal of targeting even more dilute cells. T cells were separated from a mixed-cell medium on a chip (Jurkat cells/K562 cells, 1/1000). A novel microchamber was developed to capture cells during the purification, which required a large biosample. Cell detection was demonstrated through the performance of genetic identification on the chip.

Citation

Hsu-Chao Hao, Hwan-You Chang, Tsung-Pao Wang, Da-Jeng Yao. Detection of cells captured with antigens on shear horizontal surface-acoustic-wave sensors. Journal of laboratory automation. 2013 Feb;18(1):69-76

Mesh Tags

Substances

PMID: 22496249

search → result