cGMP-dependent protein kinase I is involved in neurite outgrowth via a Rho effector, rhotekin, in Neuro2A neuroblastoma cells.

Keizo Yuasa, Takeshi Nagame, Makoto Dohi, Yayoi Yanagita, Shin Yamagami, Masami Nagahama, Akihiko Tsuji

Department of Biological Science and Technology, The University of Tokushima Graduate School, Tokushima 770-8506, Japan. yuasa@bio.tokushima-u.ac.jp

Biochemical and biophysical research communications 2012 May 4

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Although the cGMP/cGMP-dependent protein kinase (cGK) signaling is involved in the regulation of neurite outgrowth, its mechanism remains to be clarified. In this study, we identified a Rho effector, rhotekin, as a cGK-I-interacting protein. Rhotekin was also a substrate for cGK-Iα. In neurite-extended Neuro2A neuroblastoma cells, cGK-Iα and rhotekin were colocalized in the plasma membrane and extended neurites, while treatment with cGMP resulted in translocation of rhotekin to the cytoplasm. In addition, we found that cGK-Iα and rhotekin synergistically suppressed Rho-induced neurite retraction. Our findings suggest that cGK-Iα interacts with and phosphorylates rhotekin, thereby contributing to neurite outgrowth regulation. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Keizo Yuasa, Takeshi Nagame, Makoto Dohi, Yayoi Yanagita, Shin Yamagami, Masami Nagahama, Akihiko Tsuji. cGMP-dependent protein kinase I is involved in neurite outgrowth via a Rho effector, rhotekin, in Neuro2A neuroblastoma cells. Biochemical and biophysical research communications. 2012 May 4;421(2):239-44