Cocrystallization and preliminary crystallographic analysis of an inactive MaoC-like hydratase mutant with the substrate crotonyl-CoA.

Huizheng Wang, Jie Zhu, Weiwei Song, Xiuguo Zhang

Acta crystallographica. Section F, Structural biology and crystallization communications 2012 Apr 01

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MaoC-like hydratase (MaoC) is a recently identified enzyme involved in the biosynthetic pathway of polyhydroxyalkanoates (PHAs), which are completely biodegradable polymers used to produce green plastics. The inactive mutant D194N-MaoC was crystallized in the presence of the substrate crotonyl-CoA. Crystals were grown in a number of conditions, but only those produced using 20%(v/v) ethylene glycol were suitable for structural studies. Data were collected to 2.10 Å resolution using X-radiation and the crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 81.40, b = 82.58, c = 123.99 Å. © 2012 International Union of Crystallography. All rights reserved.

Citation

Huizheng Wang, Jie Zhu, Weiwei Song, Xiuguo Zhang. Cocrystallization and preliminary crystallographic analysis of an inactive MaoC-like hydratase mutant with the substrate crotonyl-CoA. Acta crystallographica. Section F, Structural biology and crystallization communications. 2012 Apr 01;68(Pt 4):483-5