Molecular epidemiology of rifampicin mono-resistant Mycobacterium tuberculosis].

Shiomi Yoshida, Kazunari Tsuyuguchi, Katsuhiro Suzuki, Motohisa Tomita, Masaji Okada, Seiji Hayashi, Tomotada Iwamoto

Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku, Sakai-shi, Osaka 591-8555 Japan. dustin@kch.hosp.go.jp

Kekkaku : [Tuberculosis] 2012 Feb

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We aimed to investigate the prevalence and possible transmission routes of rifampicin (RFP) mono-resistant Mycobacterium tuberculosis strains. Drug susceptibility testing was used to identify 15 RFP-resistant strains out of 4633 M. tuberculosis isolates. Sequencing of the rpoB gene and VNTR analysis were performed to further confirm the genetic classification. Resistance-conferring mutations in the RFP resistance-determining region (RRDR) of the rpoB gene were found in 14 of the 15 strains with phenotypic RFP mono-resistance. VNTR analysis revealed 2 clusters of 5 identical strains each. Although the community prevalence of RFP mono-resistant M. tuberculosis is low, the results of VNTR analysis suggested that rather than being recently transmitted, these strains may have been widely transmitted as latent infections in the population.

Citation

Shiomi Yoshida, Kazunari Tsuyuguchi, Katsuhiro Suzuki, Motohisa Tomita, Masaji Okada, Seiji Hayashi, Tomotada Iwamoto. Molecular epidemiology of rifampicin mono-resistant Mycobacterium tuberculosis]. Kekkaku : [Tuberculosis]. 2012 Feb;87(2):41-5