Lipid fingerprinting in women with early-onset preeclampsia: a first look.

Leandro De Oliveira, Niels Olsen S Câmara, Tatiana Bonetti, Edson G Lo Turco, Ricardo P Bertolla, Antonio F Moron, Nelson Sass, Ismael Dale Cotrim Guerreiro Da Silva

Obstetrics Department, Universidade Federal de São Paulo, Brazil. Leminas@ig.com.br

Clinical biochemistry 2012 Jul

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The aim of this preliminary study was to characterize the plasma lipid profiling of women with preeclampsia. Plasma samples of 8 pregnant women with early-onset preeclampsia and 8 normal pregnant women were evaluated. Lipids were extracted from plasma using the Bligh-Dyer protocol. The extracts were subjected to MALDI-MS. Data matrix was exported for partial least squares discriminant analysis (PLS-DA) and a parameter VIP was employed to reflect the variable importance in the discriminant analysis. The major discriminant variables were selected and underwent to Mann-Whitney U test. A total of 1290 ions were initially identified and twelve m/z signals were highlighted as the most important lipids for the discrimination of patients with preeclampsia. The identification of these differential lipids was carried out through Lipid Database Search. The main classes identified were glycerophosphocholines [GP01], glycerophosphoserines [GP03], glycerophosphoglycerols [GP04], glycosyldiradylglycerols [GL05] and glycerophosphates [GP10]. Copyright © 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Citation

Leandro De Oliveira, Niels Olsen S Câmara, Tatiana Bonetti, Edson G Lo Turco, Ricardo P Bertolla, Antonio F Moron, Nelson Sass, Ismael Dale Cotrim Guerreiro Da Silva. Lipid fingerprinting in women with early-onset preeclampsia: a first look. Clinical biochemistry. 2012 Jul;45(10-11):852-5